Why colloidal gold can be used as a marker in immunohistochemistry

Why colloidal gold can be used as a marker in immunohistochemistry

2023-02-14 16:24:15  Blog

What is colloidal golden? What is colloidal gold? Also known as gold solution, it refers to gold that has dispersed phases with diameters between 1-150nm and 1-15 mm. It is part of a heterogeneous, reddish-orange to purple-red system. Colloidal gold can be used as a marker in immunohistochemistry. The technology of colloidal gold labelling became an important immuno labelling tool over the last 10 years. Colloid gold immunoassay has been studied in biomedicine, drug detection, and other areas. Also known as "the gold solution", colloidal or colloidal, colloidal, gold is a suspended of original gold particles that are formed through the reduction of sodium into it. A colloidal layer of gold is composed of the basic gold core (atomical gold Au), and an outer layer that contains a double-ion layer. Nearly touching the gold core surface is the inner layer with negative ions (AuC12-12 -). The outer layer with ion H+ is dispersed within the colloidal solution. To maintain colloidal gold's suspended state, and to preserve its electrolyte sensitivity, colloidal golden has many characteristics.

Colloid gold preservation and identification
While it's easy to prepare colloidal golden, it can prove difficult to create high quality colloidal. Each batch of colloidal golden should be checked for quality. Particle size and uniformity are important indicators. It is possible to estimate the size and colour of gold particles by carefully observing the colloidal gold under sunlight. To estimate the amount of gold particles, you can scan it with an electron microscope l Max. It is best to use an electron microscope for the observation of prepared colloidal Gold. Some representative colloidal Gold samples will be selected for micrography. Clear and transparent colloidal Gold should be observed. Imagine that prepared colloidal golden is cloudy, or that there are floating items on its surface. If this happens, the prepared colloidal golden has more agglutination particles. Clean glassware can keep colloidal gold safe for many years. Preserving colloidal gold can be made easier by adding 0.02% NaN3 to the glassware. It can lead to bacterial growth, or even the creation of agglutination molecules if it is not stored properly. Colloidal gold labelling is not affected by small quantities of agglutination. Low-speed centrifugation can remove the agglutinated particle to increase labelling efficiency.

Colloidal gold can be used
Colloidal Gold can now be used to detect immune cells in macros. Colloidal gold has several advantages. Covalent bond formation is not required during the gold labelling procedure. It is simply the process of physical adsorption that occurs at certain ions concentrations. Most macromolecular materials can be labeled with gold. The activity of macromolecular chemicals will not alter after the labelling process. Experimental results have demonstrated that colloidal golden can be sensitive enough to match ELISA. Combining silver staining with colloidal gold will greatly improve the detection sensitivity.

Colloidal gold price
Price of colloidal silver will be affected by the size and purity of its particles. The purchase volume may also have an effect on price. Large quantities of small amounts will result in a lower price. Our company website provides information about the colloidal price.

Manufacturer of Colloidal Gold
Rboschco Technology Co. Ltd. Luoyang City (Rboschco), China is a trustworthy and quality global chemical material manufacturer and supplier. They have more than 12 years experience producing high quality chemical and nanotechnology products, such as colloidal and graphite gold, nitride and graphite powders, and 3D printing and sulfide materials. Feel free to inquire if you need high-quality colloidal gold at a reasonable price.


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